Determination of antibiotic susceptibility of bacteria isolated from wastewater in the city of Bishkek (April – June 2025)

The aim of this study was to conduct a comprehensive assessment of the microbiological contamination of wastewater in Bishkek city and to determine the antibiotic susceptibility of isolated microorganisms. Between April and June 2025, five monitoring points were examined, including the wastewater discharge area near the thermal power plant (TPP), several sites along the Big Chui Canal (BCC) in different parts of the city, and the municipal wastewater treatment facilities. A total of 15 water samples were collected and analyzed using the titration method in accordance with the standards of the European Committee on Antimicrobial Susceptibility Testing (EUCAST). The bacteriological analysis identified representatives of conditionally pathogenic microflora — Escherichia coli, Proteus mirabilis, and Enterobacter aerogenes. All isolated strains showed susceptibility to the tested antibiotics, including ciprofloxacin, ceftriaxone, imipenem, and amoxicillin/clavulanic acid. The highest level of microbial contamination was recorded near the TPP discharge area, likely due to high concentrations of organic matter and elevated temperature. Although no resistant strains were detected, the findings indicate a potential risk of antibiotic resistance emergence under the influence of climate-related factors such as rising temperatures and altered hydrological conditions. The study emphasizes the importance of regular microbiological and climate monitoring of Bishkek’s water bodies, improvement of wastewater control systems, and the development of preventive measures to curb the spread of resistant microorganisms in the context of climate change